Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | location of an allosteric activation switch in the multisubunit phosphorylase kinase complex, overview | Oryctolagus cuniculus |
Cloned (Comment) | Organism |
---|---|
two-hybrid plasmid construction of fusions containing the PhK beta subunit and its deletion mutants and expression in a yeast two-hybrid system | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of PhKbeta subunit deletion mutants | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Oryctolagus cuniculus | |
Mg2+ | - |
Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycogen phosphorylase b | Oryctolagus cuniculus | - |
ADP + phosphorylated glycogen phosphorylase b | - |
? | |
additional information | Oryctolagus cuniculus | the enzyme complex regulates glycogenolysis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
New Zealand white rabbits | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | subunit PhKalpha is autophosphorylated | Oryctolagus cuniculus |
Purification (Comment) | Organism |
---|---|
native enzyme from psoas muscle | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
psoas | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycogen phosphorylase b | - |
Oryctolagus cuniculus | ADP + phosphorylated glycogen phosphorylase b | - |
? | |
ATP + glycogen S peptide | synthetic peptide corresponding to residues 5-18 of its convertible region | Oryctolagus cuniculus | ADP + phosphorylated glycogen S peptide | - |
? | |
additional information | the enzyme complex regulates glycogenolysis | Oryctolagus cuniculus | ? | - |
? | |
additional information | subunit PhKalpha is autophosphorylated | Oryctolagus cuniculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | the enzyme forms a (alphabetagammadelta)4 complex, location of an allosteric activation switch in the multisubunit phosphorylase kinase complex, overview | Oryctolagus cuniculus |
Synonyms | Comment | Organism |
---|---|---|
PhK | - |
Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at, substrate glycogen phosphorylase b | Oryctolagus cuniculus |
8.3 | - |
assay at, substrate glycogen S peptide | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Oryctolagus cuniculus |